Out of 6 clinical skin specimens confirmed as M. PCR positive samples were all positive by LAMP detection of 6 clinical M. Results: Nested PCR was 10-fold more sensitive than the LAMP assay by serial dilution of M. Then, the sensitivity of the LAMP and nested PCR assays were assessed by M. marinum genomic DNA and confirmed the specificity of the primers. We optimized LAMP performance for detection of M. marinum infection were included in the study.
marinum strains and 6 skin samples with definite diagnosis of M. marinum in skin samples from patients with M. Our study aimed to compare the rapid diagnostic abilities of polymerase chain reaction (PCR), nested PCR and loop mediated isothermal amplification (LAMP) of detecting M. Seeking rapid and sensitive diagnostic methods for diagnosis can greatly improve patient treatment. Purpose: Culture of Mycobacterium marinum is very time-consuming, taking several weeks to produce positive results. 12 Jiangwangmiao Street, Nanjing, People’s Republic of China, Tel + 8953, Email *These authors contributed equally to this workĬorrespondence: Hongsheng Wang, Institute of Dermatology, Chinese Academy of Medical Sciences & Peking Union Medical College, No. Yumiao Feng, 1, 2, * Miaomiao Wang, 2, 3, * Haiqin Jiang, 2 Ying Shi, 2 Wenyue Zhang, 2 Hongsheng Wang 2ġDepartment of Dermatology, the Affiliated Suzhou Hospital of Nanjing Medical University, Suzhou, People’s Republic of China 2Department of Mycobacterium, Jiangsu Key Laboratory of Molecular Biology for Skin Diseases and STIS, Institute of Dermatology & Hospital of Skin Diseases, Chinese Academy of Medical Sciences & Peking Union Medical College, Nanjing, People’s Republic of China 3Department of Dermatology, the First Affiliated Hospital of Soochow University, Suzhou, People’s Republic of China
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